Acetyl triethyl citrate (ATEC), a citric acid ester used as an alternative to phthalate plasticizers, has raised concerns due to reported hepatotoxicity, lipid metabolism disruption, and embryotoxicity. However, data on human metabolites remain limited. This study aimed to identify ATEC metabolites via in vitro and in vivo approaches and establish a method for urinary quantification. In vitro experiments

The plasticizer di(2-ethylhexyl) phthalate (DEHP) is frequently detected at high environmental concentrations in the environment. Bacillus thuringiensis (Bt), used for insect control, and the soil nematode Caenorhabditis elegans (C. elegans) likely coexist in soil ecosystems. Avoidance behavior is essential for organisms to evade potential hazards and optimize survival in fluctuating environments;

2,4-D dimethylammonium is a widely used herbicide known for its environmental persistence and adverse effects on aquatic ecosystems. This study investigates the toxicological impacts of 2,4-D dimethylammonium on rice field crabs (Somanniathelphusa bangkokensis), focusing on morphological, biochemical, and histological alterations. Crabs were exposed to varying concentrations of 2,4-D dimethylammonium

Aflatoxin B1 (AFB1)-contaminated crops could serve as insect feed without affecting growth and survival of black soldier fly (BSFL), housefly (HFL), and lesser mealworm (LMW) larvae. However, the genotoxic safety of larvae reared on AFB1-contaminated substrate remains uncertain. This study assessed the genotoxic effects of larvae reared on 0, 20, and 200 µg/kg AFB1 spiked substrate through in vitro

The stingless bee Partamona helleri plays a crucial role in pollinating both native and cultivated plants. However, the intensive use of pesticides in agriculture are contributing to the decline of its population. Thiamethoxam, a systemic neonicotinoid insecticide, acts on the nervous system, however, it can also affect other non-target insect organs. This study evaluated the sublethal effects of chronic

Ametryn (AMT) and clomazone (CLZ) are widely used for weed control, often in combination. This study evaluated the effects of environmentally relevant concentrations of AMT, CLZ, and their mixtures on zebrafish embryonic-larval development and enzyme activity. Exposure to AMT (≥0.1 µg/L) and CLZ (≥36 µg/L) significantly reduced swim bladder inflation, although their mixture showed an antagonistic effect

The present study aims to investigate the impact of chlorpyrifos on the oxidative stress responses in the freshwater bivalve Lamellidens marginalis. To assess the extent of chlorpyrifos induced stress, physiological parameters including respiration and excretion rates alongside a battery of biomarkers like superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST), and lipid peroxidation

Municipal wastewaters contain a peculiar mix of rare earth elements (REEs) dominated by gadolinium (Gd). The sublethal and lethal toxicity was examined in rainbow trout exposed to a representative REE mixture (municipal waswaters) composed of Gd (105 ng/L), Ce (9 ng/L), Nd (8 ng/L), Yb (6.2 ng/L) and Dy (4 ng/L) corresponding to total REEs loading of 137 ng/L. Juveniles were exposed to a mixture of

Ivermectin has good antiparasitic activity and is heavily used in animal husbandry. However, its extensive use puts pressure on the environment. This study focused on evaluating the safety of ivermectin on the nervous system of aquatic organisms. The effects of ivermectin on the neurological development of zebrafish larvae were mainly explored. The results showed that ivermectin was able to induce

Ammonia gas (NH3) is a major hazard in animal husbandry that negatively affects animal growth and reproductive activities. Although the adverse effects of ammonia on livestock health and performance have been studied, its specific effects on the male reproductive system and its underlying biological mechanisms remain unclear. The aim of this study was to investigate the effects of ammonia exposure

Many agrochemicals disrupt redox homeostasis, yet the dynamics of oxidative stress responses in honey bees (Apis mellifera) remain insufficiently understood. This study established a controlled model to monitor ROS-related markers over time following paraquat injection, using Bayesian modeling to characterize time-dependent changes. We observed a transient rise in hydroperoxides and early fluctuation

In this study, we investigated the impact of iron-rich nanoparticles derived from different locations in the subway on the innate immune system in blood. Nanoparticles were generated from Third Rail, Rail, and Wheel materials and characterized using several techniques. The response in a human whole-blood model was analyzed using ELISA and capillary immunoelectrophoresis. All nanoparticles were iron

Pesticide use has transformed agriculture, but improper handling poses serious health and environmental risks. Brazil, a top global pesticide consumer, faces alarming unapproved usage—nearly 80 % of pesticides sold lack approval in at least three OECD countries. This study investigates genotoxic effects on farmers in Paraíba, Brazil, analyzing buccal mucosa cells for DNA and cellular damage. Using

To investigate the impact of PDYN 68-bpVNTR, BDNF rs6265, OPRD1 rs569356 and OPRM1 rs2075572 variations on the susceptibility to opioid-methamphetamine co-use, a total of 532 individuals, including opioid (n = 104), and methamphetamine (n = 166) users as well as opioid and methamphetamine co- users (n = 158) and healthy individuals (n = 104), were included. BDNF rs6265, OPRD1 rs569356 and OPRM1 rs2075572

Multiple pesticides often coexist in soil, potentially leading to interactions among their components, these may detrimentally impact soil organisms. This study assessed the potential risks posed by simultaneous exposure to atrazine (ATR) and phoxim (PHO) on enzyme and transcription levels in earthworms (Eisenia fetida). The results revealed that ATR exhibited higher acute toxicity towards E. fetida

We explored the association between exposure to air pollutant mixture and renal function. A nationwide sample of 69,066 Korean adults was analyzed. The 1-year moving averages of the concentrations of PM10, PM2.5, SO₂, NO₂, CO, and O₃ were estimated using the air pollution modeling. The outcome was estimated glomerular filtration rate (eGFR) value. Quantile-based G-computation analyses were performed

The rising losses of honey bee colonies are causing public concern regarding the species' long-term sustainability. Therefore, this study aimed to assess how pesticide exposure affects DNA damage and morphological traits of drone spermatozoa across different Apis mellifera populations, categorized by varying colony loss rates. Chromatographic analysis identified 24 pesticide residues in bee samples

Tebuconazole (TBZ) is a broad-spectrum fungicide that disrupts fungal cell membrane. Due to its extensive application in agriculture, TBZ is frequently detected in aquatic ecosystems, posing potential risks to amphibians. However, the effects of TBZ-based formulations on amphibian development remain poorly understood. The present study investigates the effects of the TBZ-based fungicide Tebucur on

Pesticides used in agriculture to control pests can also affect non-target animals, such as livestock and humans, through exposure. Pesticide residues found in animal-derived products like milk serve as evidence of exposure in mammals and may pose health risks due to bioaccumulation. Understanding the mechanisms of elimination and toxicity of pesticides is essential to minimize their negative impact

Airborne fine particulate matter (PM2.5) has been linked to neurological diseases, but its cellular and metabolic effects remain incompletely understood. This study assessed the cytotoxic and metabolic impact of PM2.5 samples from São Paulo, Brazil, on SH-SY5Y neuroblastoma cells. Even at low toxicity levels (IC10–IC30), PM2.5 organic extracts induced apoptosis, increased TNF-α secretion, and triggered

Metabolic dysfunction-associated steatotic liver disease (MASLD) affects around 30 % of the world’s population, increasing its prevalence by 50 % in the last three decades. MASLD pathogenesis is considered multiaxial, involving disturbances in the liver, adipose tissue (AT), and gut microbiome. In parallel with MASLD increasing trends, the total herbicide use has nearly tripled over the last three

Bipyridyl herbicides, like paraquat, are among the most widely used herbicides worldwide. Although the lungs are the main target organ for acute exposure to paraquat, chronic exposure to paraquat is thought to induce neurotoxicity and is one of the environmental risk factors for neurodegenerative diseases. In the last two decades, as paraquat has been banned in some regions, diquat has gradually become

Exploring the presence of endocrine disrupting compounds in marine organisms, particularly cetaceans, is crucial to evaluate contamination within the ocean's food web and the detection of synthetic hormones in cetaceans is a strong indication of anthropogenic pollution in marine ecosystems. Due to their characteristics, blubber samples are a key component in assessing the analysis of cetaceans, but

Polystyrene microplastics (PS-MPs) pose significant risks to honeybee health. However, how microplastics (MPs) adversely influence honeybee survival through the gut pathway, especially the metabolic processes, remains poorly understood. To conduct the experiment, the honeybees (Apis mellifera L.) were exposed to PS-MPs (0.5 μm and 5 μm) at environmental concentrations of 25 mg/L and 50 mg/L for 21

Endotoxin-induced toxicity could increase with previous exposure to sensitizing agents like endocrine disruptors (EDs). We studied the effects on the reproductive axis on adult male rat pups exposed to BPA since gestation day (GD) 1 to final lactation and its response to LPS before sacrifice. The decrease in GnRH release caused by LPS and BPA was more pronounced in the BPA + LPS group compared to the

Chlordecone (CLD) is an organochlorine pesticide that is highly resistant in the environment. This compound and its metabolite chlordecol (CLD-OH) still can be found in the French West Indies, after being banned 30 years ago. The novelty of this work lies in evaluating the toxicity of CLD-OH compared to CLD and examining the effects of these compounds on nuclear receptor (PXR, PPARα, and CAR) and metabolism-related

4,4’-oxydianiline (ODA) is an environmental aromatic amine reasonably anticipated to be a human carcinogen. We investigated ODA genotoxicity and oxidative stress modified by N-acetyltransferase 2 (NAT2) genetic polymorphism in yeast and DNA repair deficient (UV5) Chinese hamster ovary cells (CHO) expressing human NAT2*4 (rapid acetylator allele) or human NAT2*5B (slow acetylator allele) and in cryopreserved

Pesticides pose significant threats to pollinators, and honey bees are frequently exposed through foraging and beekeeping practices. We assessed honey bee pesticide exposure by analyzing 92 pesticide residues in honey from 30 hobbyist apiaries across Massachusetts, along with store-bought honey and commercial wax foundation. For all samples, we calculated the risk of multiresidue toxicity to honey

This study assessed the effects of TCDD, two PCB mixtures (Clophen A50 and Aroclor 1254), and field extracts from marine sediments and swimming crab tissues on early-life development in Caenorhabditis elegans. Gravid nematodes were exposed on agar, and isolated eggs and larvae were tested in solution. Larval development was evaluated after 72 hours. Reporter gene assays (DR-CALUX) were also used to

This study investigated the multilevel effects of environmentally relevant concentrations of fluoxetine on serotonergic signaling and enteric neurogenesis in early zebrafish larvae (Danio rerio). To this end, zebrafish were exposed to various concentrations of fluoxetine for four days, from the 1,000-cell stage to 4 days post-fertilization (dpf).Following exposure, whole larvae were subjected to molecular

ABC transporters are important for excretion of xenobiotics and metabolites into urine. They are sensitive to metallic pollutants like cadmium, mercury, zinc, or arsenic. Here, we show that copper (Cu(ATSM)) stimulates ABC transporter-mediated export in isolated renal proximal tubules from Atlantic killifish (Fundulus heteroclitus) with main focus on Mrp2. Transporter stimulation was reduced by cycloheximide

Polycystic ovarian syndrome (PCOS), which causes hormonal imbalance, inflammation, and metabolic disorders, requires several treatments. This study aimed to examine the Isatin-linked pyrazole K1 derivative's effectiveness in PCOS induced by environmental contaminants such as triclosan, specifically assessing its biochemical, metabolic, and reproductive impacts. Isatin-linked pyrazole K1 derivative

Lithium has long been the primary treatment for bipolar disorder and shows promise for managing other neurological and psychiatric conditions. We previously identified the Lithium-inducible SLC6 transporter (List) in Drosophila melanogaster as a gene significantly upregulated in response to lithium chloride supplementation. List encodes a putative amino acid transporter belonging to the Na⁺-dependent

The environmentally relevant aquatic pollution is associated with the mixtures of xenobiotics, each in the low, picomolar to micromolar concentrations. Among these substances, the combinations of pharmaceuticals and microplastics (MP) have become an increasingly serious threat. The objective of this study was to track the specific and multi-stress responses of swollen river mussels (Unio tumidus) to

The concentration of heavy metals (HMs) in human hair is a bioindicator of environmental pollution over a long period from worker, including the informal worker at landfills. A landfill is where final-stage refuse is deposited, transported, and disposed of solid waste from urban areas. No study has been conducted on heavy metals in landfills and their health impact on informal workers. This study aims

Beauty salons are unique indoor environments where elevated PM10 levels may arise from frequent use of personal care and cosmetic products, potentially exposing salon workers and clients to harmful chemicals. This study aimed to assess the biological effects of PM10 collected indoors and outdoors of a beauty salon, as well as the mutagenic potential of PM10-bound polycyclic aromatic hydrocarbons (PAHs)

Mixing several agrochemicals, e.g., insecticides, fungicides, and herbicides, is a common practice by farmers, enhancing the economic and efficacy of a single application. Their effects on non-target organisms are not routinely evaluated during the authorisation of single products. A field study was conducted in Germany to assess the performance and exposure level of red mason bees after application

Propylene glycol ethers are increasingly used as organic solvents of choice in domestic products and industrial manufacturing. However, little is known about their potential neurotoxic effects. In the present study, we therefore evaluated the acute toxic and behavioral effects of propylene glycol ethers using zebrafish larvae as a vertebrate model. Studied endpoints included viability, motor behavior

Limited knowledge exists on persistent organic pollutants (POPs), including polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs), in mother-child pairs from Northern Finland. This study examines plasma PCB and OCP concentrations and their determinants. Blood plasma concentrations of 13 PCBs and 6 OCPs were measured in mothers and in cord samples from the NUGEN birth cohort (N = 102

This study showed that single cyanobacterial metabolites had various effects on the tested parameters. Among them, only cylindrospermopsin was lethal to the animals; cylindrospermopsin was also the most potent inhibitor of the remaining parameters. Cylindrospermopsin in binary mixtures with the other tested metabolites displayed antagonistic or additive effects for survival, movement, food uptake and

Mito-toxic fungicides used in crop protection negatively affect pollinating insects. The fungicide formulation Pristine® (ai: 25.2 % boscalid, 12.8 % pyraclostrobin) induces precocious foraging, reduced lifespan, impaired homing abilities, and reduced body size at field-relevant concentrations. However, the underlying physiological mechanisms for these outcomes are poorly understood. To assess the

The use of pesticides to enhance crop yields remains prevalent despite persistent concerns about their potential harmful side effects. Moreover, most cell viability studies are conducted in growth media and oxygen concentrations that do not accurately reflect the in vivo conditions in tissues, and therefore may influence the outcomes of toxicity assessments. The aim of the study was to examine how

Dimethylmonothioarsinic acid (DMMTAV), a potent toxic metabolite of arsenic, exhibits higher cytotoxicity than other arsenicals. This study investigates its influence on NAD(P)H:quinone oxidoreductase (NQO1) regulation in C57BL/6 mice and Hepa-1c1c7 cells. Mice were administered DMMTAV (6 mg/kg, IP) with or without TCDD (15 µg/kg, IP), and hepatic and extrahepatic tissues were analyzed for NQO1 expression

This study aimed to assess the risk of pesticides on bee pollinators and propose strategies to conserve entomophilous angiosperm species in the Ndop wetlands. Results showed that the applied pesticide doses were often excessive, increasing the exposure toxicity ratios (ETR). Among the pesticides, insecticides posed the highest risk to bee pollinators, with Emamectin benzoate (ETR=591.4) presenting

Rare Earth Elements, particularly gadolinium (Gd), are increasingly used in industrial and medical applications, leading to their dispersion in aquatic environments. This study examines the ecotoxicological effects of Gd on aquatic organisms across different trophic levels in freshwater and marine ecosystems. Using standardized bioassays, the impact of Gd was assessed on Aliivibrio fischeri, Raphidocelis

5-hydroxy-N,N-dimethyltryptamine (5-OH-DMT), known as bufotenine, is proven to have psychoactive effects in high concentrations. Duttyphrynus melanostictus, which produces bufotenine, has migrated to the city of Toamasina in Madagascar, thus, the determination bufotenine’s levels in the species’ samples is necessary. This study aimed to quantify bufotenine in eggs, tadpoles and toad’s skin samples

We present a review about histological sub-lethal effects due to anthropogenic contaminants on the midgut of bees and other aculeate hymenopterans. Contaminant types, damage types, and methodology were extracted and summarized from 74 published articles, and then quantitatively analyzed. We found that the Western honeybee (Apis mellifera) is by far the most widely used model. Contaminants have largely

Cardiovascular disease (CVD) is a leading cause of death that is sexually dimorphic. This study used an ovine model to investigate whether maternal exposure to an environmental chemical (EC) mixture (biosolids) prior to and throughout pregnancy, affected offspring cardiovascular (CV) structure and function in adulthood. CV function of male and female offspring from ewes grazed on either conventionally

Heart rate variability (HRV) of 10 participants was assessed to evaluate the impact of exposure to first- and second-hand smoke from heat-not-burn (HnB) products and conventional cigarettes on cardiovascular health and the autonomic nervous system. The nicotine and cotinine concentrations in the urine of smokers and non-smokers exposed to this smoke were also measured. Nicotine levels in the urine

We previously identified the trimethylsulfonium ion (TMS) in human urine and highlighted its potential as a novel H2S biomarker but observed significant inter-individual variability in its urinary excretion. In this work we investigate the contribution of genetic factors to this variability in a group of European subjects (n = 100) from the BioPersMed cohort. Urinary TMS concentrations displayed two

Nanoparticles of titanium dioxide (TiO2) and zinc oxide (ZnO), have great demand and commercial use. Their presence in water reservoirs constitute a potential to produce adverse effects on crustaceans. We used two species of crustaceans: the cladoceran Chydorus sphaericus, and the ostracod Cypridopsis cf. vidua. Cultures were kept in a bioclimatic chamber at 20°C and 25°C respectively. Model organisms

The pacemaker channel (HCN) is responsible for electrical activity in a wide range of excitable cells, including those of invertebrates. Using Xenopus oocytes and HEK cell, we show here that HCN-channel from Apis mellifera is activated by hyperpolarization, modulated by cAMP, and blocked by cesium. Its PNa/PK relative permeability is 1:3, and its unitary conductance is 1.5 pS, which is similar to that

Recent research has highlighted the crucial role of immune regulation in lung function impairment due to exposure to hazardous materials. This study aimed to identify dynamic network biomarkers for lung function damage caused by hexavalent chromium inhalation exposure, using immune-related indicators in blood. An 11-year follow-up longitudinal study was conducted on a population occupationally exposed

Pesticide exposure can cause various adverse effects in humans, with children being particularly susceptible. Such exposure leads to neurological, immunological, respiratory, and genetic damage, primarily by generating reactive oxygen species (ROS). The increase in ROS induces lipid peroxidation (LPO) and the formation of hydroxyl radicals, which generate DNA adducts. This study involved children aged

Bees play a crucial role as pollinators, significantly contributing to ecosystem health. However, they face growing threats from human activities. This study uses biomarkers to evaluate the health status of Melipona mandacaia, a stingless bee native to the Caatinga biome, as indicators of anthropogenic stress. Bees were collected from the unique Caatinga biome, which had no recorded human pressure

Microplastics (MPs) in aquatic environments can have toxic effects on various organisms, including fish. This study exposed Pseudobagrus fulvidraco to polyethylene MPs at 0, 10 mg/L (approximately 9.50 ×108 particles/L), 20 mg/L (approximately 1.9 ×109 particles/L), 5000 mg/L (approximately 4.75 ×1011 particles/L), and 10,000 mg/L (approximately 9.50 ×1011 particles/L) concentrations for 96 h. At relatively

Carbamazepine (CBZ) is a pharmaceutical commonly used in the treatment of epilepsy and bipolar disorder and has been detected in different aquatic ecosystems worldwide. Considering its possible role in altering nervous system and reproduction, this study aimed to evaluate the effects of CBZ on molecular and cellular biomarkers of the teleost Astyanax lacustris. Results demonstrated that CBZ, in environmentally

Methylmercury (MeHg) exposure poses significant risks, especially for pregnant women in Indonesia, where fish consumption is a common dietary habit. This study used a physiologically based toxicokinetic model and reverse dosimetry to estimate MeHg exposure levels and assess health risks in pregnant Indonesian women. The model was validated against biomarker data, with observed MeHg blood levels of